This is a case report of a 63-year-old male patient who presented with rhinophyma of 17 years duration.Several medical treatments were applied previously, with no response or poor improvement. We presentour experience by combining the Versajet™ Hydrosurgery System and ReCELL® in a heavy smokerpatient, which led to a good aesthetic outcome.With the combined technique, we did not encounter any difficulties either within the operation or inthe follow-up period. We obtain

Objective: The aim of the present study was to evaluate theviability and proliferative capacity of adipose-derived stem cellsobtained by laser-assisted liposuction (LAL).Methods: Fat tissue was obtained from 7 male patients treatedsurgically for gynecomastia. On one side, harvesting was madebefore LAL, while it was implemented after LAL on thecontralateral side. Viability, cell surface antigens, pluripotency,and apoptosis were assessed and compared in these samples.Results: Cells harvested before and after LAL did not exhibit anysignificant difference in terms of surface cell markers. Number of viablestem cells was lower initially after exposure to laser, while thisdifference was reversed at the end of 72 hours. Genetic indicators ofcellular differentiation were similarin both groups. Apoptosisindicatorswere increased remarkably after laser exposure in the first 24 hours, butthis increase was absent 72 hours after LAL procedure.Conclusion: The authors’ results have promising clinical relevancesince mesenchymal stem cells harvested during LAL havemaintained appropriate cellular features to be used forautologous fat transfer and fat grafting.

Background: The aim of this study was to generate skin tissue using adiposetissue-derived mesenchymal stem cells.Methods: Thirty Wistar albino rats were used. A 2-cm-diameter full-thicknessskin defect on the back of each rat was formed. A secondary wound healingmodel was constituted in group 1, fibrin matrix only was applied in group 2,a keratinocyte-coated fibrin matrix was applied in group 3, an adipose tissuederived mesenchymal stem cell–seeded fibrin matrix was applied in group 4,and a keratinocyte-coated and adipose tissue-derived mesenchymal stem cell–seeded fibrin matrix was applied to the defects in group 5.Results: A similar form of wound healing, with contraction from the edges andulceration at the center, was observed in groups 1, 2, and 3. The wound contraction was reduced in group 4, and the epidermis was creeping from the surrounding tissue but with some ulcerations in the central part of the wounds. Ingroup 5, the defect area was almost totally epithelialized, with minimal woundcontraction. By microscopic analysis, significant increases in the collagen volume ratios and vascular volume ratios were determined in groups 4 and 5.From the fluorescent micrographs, fibroblastic differentiation and extracellularmatrix synthesis, endothelial differentiation of stem cells, and neoangiogenesisand epithelium derived from marked keratinocytes were observed in group 5.Conclusion: Formation of the adipose tissue-derived mesenchymal stem cell–seeded and keratinocyte-coated autologous fibrin scaffold