Generation of Skin Tissue Using Adipose Tissue-Derived Stem Cells

Background: The aim of this study was to generate skin tissue using adiposetissue-derived mesenchymal stem cells.Methods: Thirty Wistar albino rats were used. A 2-cm-diameter full-thicknessskin defect on the back of each rat was formed. A secondary wound healingmodel was constituted in group 1, fibrin matrix only was applied in group 2,a keratinocyte-coated fibrin matrix was applied in group 3, an adipose tissuederived mesenchymal stem cell–seeded fibrin matrix was applied in group 4,and a keratinocyte-coated and adipose tissue-derived mesenchymal stem cell–seeded fibrin matrix was applied to the defects in group 5.Results: A similar form of wound healing, with contraction from the edges andulceration at the center, was observed in groups 1, 2, and 3. The wound contraction was reduced in group 4, and the epidermis was creeping from the surrounding tissue but with some ulcerations in the central part of the wounds. Ingroup 5, the defect area was almost totally epithelialized, with minimal woundcontraction. By microscopic analysis, significant increases in the collagen volume ratios and vascular volume ratios were determined in groups 4 and 5.From the fluorescent micrographs, fibroblastic differentiation and extracellularmatrix synthesis, endothelial differentiation of stem cells, and neoangiogenesisand epithelium derived from marked keratinocytes were observed in group 5.Conclusion: Formation of the adipose tissue-derived mesenchymal stem cell–seeded and keratinocyte-coated autologous fibrin scaffold

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